Nucleotide sequence of the restriction fragments hind l and hind m of sv40 dna. Sv40 large t antigen functions in dna replication and. The determination of the total 5,224 basepair dna sequence of the virus sv40 has enabled us to locate precisely the known genes on the genome. Dna nucleotide sequencing analysis of topo i cleavage sites on sv40 dna. In many cases, the sequence data is segregated into directories for each chromosome. Within that directory a readme file will describe the various files available.
Construction of the total sequence of ecoriig fragment of sv40 dna. Complete genomic sequence analysis of a new sv40 isolate. Nucleotide sequence of the restriction fragments hind l. The circular sv40 genome is found in the cell as a minichromosome wound. Duplication of the simian virus 40 sv40 genome is the best understood. This resource provides viral genome sequence data and related information. Nucleotide sequence of a fragment of sv40 dna that contains the origin of dna. Sv40 dna replication requires interactions between proteins provided by the host cell, the viruscoded a protein and a viral sequence of. The genome of a new sv40 strainsvimb isolated from a rhesus monkey was completely sequenced and compared with other isolates.
Dna sequence profiles of sv40 regulatory regions detected in. Locate the directory for your organism of interest. Nucleotide sequence of a fragment of sv40 dna that contains the origin of dna replication and specifies the 5 ends of early and late viral rna. Complete genome sequence of a 2019 novel coronavirus sars.
Simian virus 40 sv40 has served as a useful model system for numerous studies in. For sv40 replication, the first essential step is the assembly of an ltag double hexamer at the origin dna that will subsequently melt the origin dna to initiate fork unwinding. See the readme file in that directory for general information about the organization of the ftp files. To obtain this annotated dna sequence with restriction sites, please download. The determination of the total 5224 basepair dna sequence of the virus sv40 has enabled us to locate precisely the known genes on the genome. Inhibition of sv40 replication in simian cells by specific. With the viral dna sequence in hand and new restriction. We generated a consensus sequence of 29,811 bp with no gap and high average coverage 77,000. The viral genome is organized as chromatin, which has many structural features in common with cellular chromatin.
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